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Genetic diversity among natural populations of Ottelia acuminata (Gaghep.) Dandy revealed by ISSR

发布时间:2010-04-29点击数:2440

论文题目:Genetic diversity among natural populations of Ottelia acuminata (Gaghep.) Dandy revealed by ISSR

作者:H. Y. Zhang, K. Tian1*, Y. Yu, L. Y. Li and Y. M. Yang

关键词: Ottelia acuminate, ISSR, genetic diversity, DNA polymorphism.

刊物名称:African Journal of Biotechnology

卷期:Vol. 8 (22), 16 November, 2009

页: pp. 6089-6093

摘要:Ottelia acuminata (Gagnep.) Dandy, an aquatic species of the Hydrocharitaceae, is endemic to China. A performance comparison of genetic diversity of 4 natural populations was conducted to investigate whether or not water pollution in their habitats has anything to do with this species being endangered.A total number of 120 O. acuminate accessions were analyzed, by amplification of their DNAs with 15 primers (ISSR). Thirteen primers were scored and 214 bands were detected, of which 170 were polymorphic (79.44%). The results showed that the genetic indices in polluted Jian Lake group were always the smallest ones, when compared with those of the other groups. It indicated that the polluted water did affect the genetic diversity of O. acuminate populations. And ISSRs seemed to be effective tools for detecting genetic variation among O. acuminate geographical groups.

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